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RHC-200ATC

用來(lái)測(cè)量人或動(dòng)物的尿蛋白,血清含量, 操作方便,精確度高

GO-BL2型大劑量食用油灌裝機(jī) 10升色拉油灌裝機(jī) 20L灌油機(jī)

 【中泰機(jī)械,品質(zhì)卓越】鄭州中泰機(jī)械專業(yè)生產(chǎn)熱收縮包裝機(jī)、全自動(dòng)液體灌裝機(jī)、全自動(dòng)膏體灌裝機(jī)、灌裝生產(chǎn)線、塑料封口機(jī)、電磁感應(yīng)鋁箔封口機(jī)、軟管封尾機(jī)、自動(dòng)打包機(jī)、日期打碼機(jī)、封蓋機(jī)、貼標(biāo)機(jī)等,可定做大中小型包裝設(shè)備。

名稱:時(shí)控式油類灌裝機(jī)型號(hào):GO-BL2型

·應(yīng)用范圍:本機(jī)型適用于機(jī)油、潤(rùn)滑油、食用油等行業(yè)的粘稠液體的定量灌裝。· 性能特點(diǎn):該機(jī)結(jié)構(gòu)合理,性能可靠,定量準(zhǔn)確,調(diào)整簡(jiǎn)易(任意調(diào)節(jié)灌裝量)外殼及與灌裝物接觸部分均由不銹鋼制成(標(biāo)準(zhǔn)型不銹鋼材料為304,也可定制316材質(zhì)的機(jī)型)。·工作原理:采用齒輪泵(螺桿泵,轉(zhuǎn)子泵)持續(xù)供料,經(jīng)過(guò)溢流穩(wěn)壓維持油路恒定壓力,控制灌裝時(shí)間實(shí)現(xiàn)不同計(jì)量的灌裝,灌裝開(kāi)始時(shí)先大計(jì)量,高速度灌裝。臨近目標(biāo)灌裝量時(shí),回流閥開(kāi)啟,使一部分料液流回原料罐,從而減慢灌裝頭流速,避免料液溢出容器。·顆粒包裝機(jī) 真空包裝機(jī) 熱收縮包裝機(jī)

技術(shù)參數(shù)電源電壓:AC380V灌裝精度:+1%主機(jī)功率:1.5KW氣源壓力:0.4~0.8Mpa灌裝速度:2.2~2.5秒/升(豆油)外形尺寸:700*730*1380(mm)<!-----中泰機(jī)械,打造中國(guó)灌裝機(jī)行業(yè)第一品牌。-!/>--- | 熱線: | 地址:鄭州市未來(lái)路866號(hào) | --- “選擇中泰機(jī)械 = 選擇性價(jià)比!”登臨官網(wǎng),更多精彩→ www.bzj8.com www.jixie126.com www.baowen114.com中泰機(jī)械設(shè)備有限公司生產(chǎn)各種包裝設(shè)備,各種加工機(jī)械設(shè)備齊全、技術(shù)實(shí)力雄厚、企業(yè)內(nèi)部管理嚴(yán)密、生產(chǎn)效率高;本公司生產(chǎn)的包裝機(jī)械品種多,規(guī)格齊全,并能承接各種特殊規(guī)格需求的產(chǎn)品定做。

 

 

該公司產(chǎn)品分類: 包裝相關(guān)設(shè)備

CC-112A透明槽加熱型恒溫水浴

    CC-112A透明槽加熱型恒溫水浴可以方便觀察浴槽中的變化。工作溫度為100℃,接泵接口可以用于外循環(huán),溫度穩(wěn)定性符合DIN12876標(biāo)準(zhǔn)。    CC-112A透明槽加熱型恒溫水浴技術(shù)指標(biāo)    內(nèi)槽體積:12L;    加熱功率:2KW;    溫度范圍:室溫~100℃    開(kāi)口尺寸(mm):302*165    外形尺寸(mm):333*360*335

    訂貨號(hào):2001.0004.04

     

DDG-2090A型廠價(jià)供應(yīng) DDG-2090A型工業(yè)電導(dǎo)率儀 上海博取儀器公司

 

 

 

 
DDG-2090A型工業(yè)電導(dǎo)率儀
DDG-2090A電導(dǎo)率儀是在性能的基礎(chǔ)上簡(jiǎn)化了 ,從而具有了特別強(qiáng)的價(jià)格優(yōu)勢(shì)。清晰的顯示、簡(jiǎn)易的操作和優(yōu)良的測(cè)試性能使其具有很高的性價(jià)比?蓮V泛應(yīng)用于火電、化工化肥、冶金、環(huán)保、制藥、生化、食品和自來(lái)水等溶液中電導(dǎo)率值的連續(xù)監(jiān)測(cè)。
儀器特點(diǎn):
微機(jī)化:采用高性能CPU芯片、高精度AD轉(zhuǎn)換技術(shù)和SMT貼票技術(shù)完成多參數(shù)測(cè)量、溫度補(bǔ)償、量成自動(dòng)轉(zhuǎn)換,精度高,重復(fù)性好。
性:觸摸式按鍵,無(wú)開(kāi)關(guān)旋鈕和電位器。
自動(dòng)轉(zhuǎn)換測(cè)量頻率:避免電極極化,提高測(cè)量精度。
報(bào)警功能:報(bào)警信號(hào)隔離輸出,報(bào)警上,下限可任意設(shè)定,報(bào)警滯后撤銷。
網(wǎng)絡(luò)功能:隔離的電流輸出和RS485或RS232通訊接口
隔離電流輸出:電流對(duì)應(yīng)電導(dǎo)率的輸出上、下限可任意設(shè)定
上海博取儀器有限公司/水質(zhì)監(jiān)測(cè)儀器儀表廠家/在線電導(dǎo)率儀/工業(yè)電導(dǎo)率儀/DDG-2090A型工業(yè)電導(dǎo)率儀
 
技術(shù)指標(biāo):1.顯示方式:LED(數(shù)碼管)顯示、高亮度,醒目直觀,可視距離遠(yuǎn)
2.測(cè)量范圍:(0~20000)μS/cm 溫度:0~99.9;
3.電極常數(shù) 0.0120μS/cm(配K=0.01電極); 
0.1200μS/cm(配K=0.1電極);
1.02000μS/cm(配K=1.0電極);
1020000μS/cm(配K=10.0電極);
4.分辨率:       0.001μS/cm.0.1
5.電極常數(shù):     0.001~15.0cm-1間有軟件設(shè)定
6.溫補(bǔ)范圍:     自動(dòng)0~99.9,手動(dòng)0~99.9,25折算
7.精度:           ±1.0%FS,±0.3
8.電流隔離輸出:  4~20 mA(負(fù)載<750 Ω );
9.報(bào)警繼電器:AC220V 、3A;
10.通訊接口;:隔離RS485
11.電源:AC220V±22V ,50Hz±1Hz   DC24V
12.防護(hù)等級(jí):IP65;
13.工作條件:環(huán)境溫度060 相對(duì)濕度<90%
14.掉電保存:﹥10年;重量、尺寸:0.8 kg、96×96×110mm
15.開(kāi)孔尺寸:92×92 mm;二次表安裝方式:開(kāi)孔式
電極安裝方式:流通式/沉入式/法蘭式/管道式
表準(zhǔn)配置:二次表一臺(tái),電極一支
訂貨指南:
1.        須告知所配電極的常數(shù)
2.        電極電纜線的長(zhǎng)度,不做說(shuō)面按5m標(biāo)配
3.        電極的安裝方式
該公司產(chǎn)品分類: COD分析儀 氟離子檢測(cè)儀 磷酸根/硅酸根分析儀 鈉度計(jì) 酸堿濃度計(jì) 濁度儀 余氯分析儀 電導(dǎo)率儀 PH計(jì) 溶氧儀

WNZ-200微機(jī)控制扭轉(zhuǎn)試驗(yàn)機(jī)200N.m

簡(jiǎn)介:主要用于金屬或非金屬材料進(jìn)行扭轉(zhuǎn)強(qiáng)度測(cè)試,也可進(jìn)行各種零件,構(gòu)件抗扭強(qiáng)度試驗(yàn)。該機(jī)由主機(jī),控制系統(tǒng)和測(cè)量單元組成,可進(jìn)行扭矩控制,扭角控制雙閉環(huán)控制。軟件系統(tǒng):軟件采用虛擬儀器測(cè)試技術(shù),具有試驗(yàn)控制、數(shù)據(jù)采集、數(shù)據(jù)分析、編制報(bào)告等功能。主要技術(shù)指標(biāo): 扭矩測(cè)量范圍(Nm): 4-200扭轉(zhuǎn)測(cè)量精度: ≤±1%扭角顯示分辨率: 0.025°主動(dòng)夾頭轉(zhuǎn)速(°/min): 4~500 加載速度(Nm/min): 0.1~100扭轉(zhuǎn)方向: 正、反兩個(gè)方向夾頭間距(mm): 0~600 可調(diào)節(jié)

57320-USupelco SPME 手動(dòng)萃取頭套裝2

 
Supelco 固相微萃。SPME)簡(jiǎn)介:美國(guó)Supelco公司專利產(chǎn)品-固相微萃取(Solid Phase Micro Extraction)1994年獲美國(guó)匹茲堡分析儀器會(huì)議R&D100項(xiàng)革新大獎(jiǎng),是一種應(yīng)現(xiàn)代儀器的要求而產(chǎn)生的樣品前處理新技術(shù),幾乎克服了以往一些傳統(tǒng)樣品處理技術(shù)的所有缺點(diǎn),集采樣、萃取、濃縮、進(jìn)樣于一體,便于攜帶,真正實(shí)現(xiàn)樣品的現(xiàn)場(chǎng)采集和富集,能夠與氣相、氣相-質(zhì)譜、液相、液相-質(zhì)譜儀聯(lián)用,有手動(dòng)或自動(dòng)兩種操作方式,讓更多的分析工作者從重復(fù)、煩瑣的操作中解脫出來(lái)。廣泛應(yīng)用于環(huán)保及水質(zhì)處理、臨床藥理、公安案件分析、制藥、化工、國(guó)防等領(lǐng)域。固相微萃取(SPME)非常小巧,狀似一只色譜注射器,由手柄(Holder)和萃取頭或纖維頭(Fiber)兩部分構(gòu)成。萃取頭是一根外套不銹鋼細(xì)管的1cm長(zhǎng)、涂有不同色譜固定相或吸附劑的熔融石英纖維頭,纖維頭在不銹鋼管內(nèi)可自由伸縮,用于萃取、吸附樣品;手柄用于安裝或固定萃取頭,可永久使用。固相微萃。SPME)的典應(yīng)用:表面活性劑環(huán)境水樣食品、香精、香料法庭樣品血、尿和體液中藥物聚合物和固樣中痕量雜質(zhì)(頂空方式)藥物中殘留溶劑氣體硫化物及揮發(fā)物(VOC
該公司產(chǎn)品分類: 水質(zhì)分析儀/多參數(shù)水質(zhì)分析儀 生化試劑 實(shí)驗(yàn)室儀器 燒杯 實(shí)驗(yàn)室用具 色譜耗材及配件 樣品前處理儀器及耗材

TTD-15-03PAC投加泵TTD-12-03電磁計(jì)量泵代理銷售

絮凝劑投加泵TTD-15-03電磁計(jì)量泵代理銷售

PAC投加泵TTD-12-03電磁計(jì)量泵代理銷售

水泥助劑添加泵TTD-10-04電磁泵選型銷售

阻垢劑TTD-06-07投加泵計(jì)量泵

緩蝕劑添加泵TTD-03-07德國(guó)TT計(jì)量泵

氫氧化鈉加藥泵TTD-02-07品牌銷售

鹽酸硝酸計(jì)量泵TTD-01-07經(jīng)銷代理

耐腐蝕定量泵TTD-0.5-07小流量計(jì)量泵

 

德國(guó)TT計(jì)量泵分為四大系列TTD手動(dòng)調(diào)節(jié)流量系列 (藍(lán)色)TTM接受脈沖信號(hào)制動(dòng)調(diào)節(jié)流量系列 (淺綠色)TTA接受0/4—20mA信號(hào)自動(dòng)調(diào)節(jié)流量系列(深灰色)TTG機(jī)械隔膜泵系列

TT計(jì)量泵的優(yōu)點(diǎn):1、電磁鐵散熱:電磁鐵運(yùn)行溫度能達(dá)到100攝氏度以上,高溫下電磁線圈磁力減小、剩磁增加。這些因素都會(huì)產(chǎn)生“泵無(wú)力”。特殊的散熱翅片和散熱陶瓷應(yīng)用這一問(wèn)題。2計(jì)量:?jiǎn)蜗蜷y 、單向性雙球防回流。停機(jī)不回留。3、數(shù)字顯示屏。

4、按鍵調(diào)節(jié)。

5、手動(dòng)調(diào)節(jié)流量、數(shù)字面板顯示防腐塑料外殼、散熱陶瓷技術(shù)專用防腐蝕固定螺釘全PTFE隔膜、全四氟軟質(zhì)閥體IP55防護(hù)等級(jí)多種材質(zhì)的泵頭可選:   PPPVC,PVDF,SS316雙球單向閥、PTFE墊片頻率調(diào)節(jié):0-100%重復(fù)精度1%以下。

絮凝劑投加泵TTD-15-03磁計(jì)量泵代理銷售

TTD計(jì)量泵產(chǎn)品說(shuō)明介紹參數(shù)

德國(guó)TT電磁隔膜計(jì)量泵產(chǎn)品介紹

計(jì)量泵TTD-03-07型號(hào)國(guó)內(nèi)經(jīng)銷商圖片

德國(guó)TTBEST計(jì)量泵常規(guī)型號(hào):

TTD-0.5-07,TTD-01-07TTD-02-07,TTD-03-07TTD-06-07

TTD-10-04 ,TTD-12-03TTD-15-03,TTD-20-03TTD-30-03,TTD-50-02

型號(hào)

流量

L/H

壓力

BAR

沖程頻率(N/M

M

KG

W

 

TTD-0.5-07

0.5

7

120

2

2.5

16

220VAC 50HZ

TTD-01-07

1

7

120

2

2.5

16

220VAC 50HZ

TTD-02-07

2

7

120

2

2.5

16

220VAC 50HZ

TTD-03-07

3

7

120

2

2.5

16

220VAC 50HZ

TTD-06-07

6

7

160

2

2.5

16

220VAC 50HZ

TTD-10-04

10

4

200

2

2.5

16

220VAC 50HZ

TTD-12-03

12

3

200

2

2.5

16

220VAC 50HZ

TTD-15-03

15

3

200

2

2.5

16

220VAC 50HZ

TTD-20-03

20

3

200

2

4.5

60

220VAC 50HZ

TTD-30-03

30

3

200

2

4.5

60

220VAC 50HZ

TTD-50-02

50

2

200

2

4.5

60

220VAC 50HZ

1. 防腐的塑料外殼(ABS+聚碳);2. IP65的防護(hù)等級(jí);3. 耐腐蝕的泵頭:PP,PVC,PTFESS 316;4. 耐腐蝕的隔膜:全PTFE隔膜,特殊的工藝結(jié)構(gòu),不壞損;5. 雙球單向閥:密封效果加倍;6. 直觀醒目的操作界面:LED數(shù)碼管顯示當(dāng)前工作頻率,更精確,重復(fù)精度更高;7. 更寬范圍的電源設(shè)計(jì):180-250VAC 50-60HZ 更適用于中國(guó)的國(guó)情;8. 強(qiáng)大的控制功能9. 可附加液位或遠(yuǎn)程控制功能

該公司產(chǎn)品分類: 塞瓦計(jì)量泵 新道茨NEWDOSE 日本安智邁計(jì)量泵 德普羅名特計(jì)量泵 SDP斯得浦計(jì)量泵 美帕斯菲達(dá)計(jì)量泵 意大利EMEC愛(ài)米克計(jì)量泵 TTbest精密計(jì)量泵 意大利道茨計(jì)量泵 意大利SEKO計(jì)量泵 米頓羅計(jì)量泵 力高計(jì)量泵 愛(ài)力浦計(jì)量泵 計(jì)量泵

LYCN/LWY-84B控溫式遠(yuǎn)紅外消煮爐(碳化硅爐體/20孔),專業(yè)生產(chǎn)控溫式遠(yuǎn)紅外消煮爐(碳化硅爐體/20孔)

一。控溫式遠(yuǎn)紅外消煮爐技術(shù)要求1、爐 孔 溫 度 調(diào) 控 范 圍 :室溫至450℃。2、單孔溫度與平均溫度偏差 :不大于20℃ 。3、 升 溫 時(shí) 間 :滿功率加熱,室溫升至450℃,不大于30分鐘。4、消 化 樣 品 數(shù) 量 : 20個(gè)/次 或12個(gè)/次 或35個(gè)/次5、消 化 管 尺 寸 :φ28×230mm、φ40×250mm、φ23×230mm;6、消化容量管容積:100ml±0.25ml 250ml±0.25ml 50ml±0.25ml7、電源 ;電壓:220V±10%,50HZ;8、最大功率:2000瓦 。9、絕緣電阻 :任一端對(duì)機(jī)殼不低于5MΩ 10、外形尺寸:480×305×180)mm 11、重量:不大于20kg 。二。控溫式遠(yuǎn)紅外消煮爐使用方法 1、正常工作:插上電源插頭,本機(jī)進(jìn)入待機(jī)狀態(tài),輕觸“電源”開(kāi)關(guān)一次,“電源”指示燈亮,數(shù)碼管顯示已存設(shè)定好的溫度值一秒鐘后,自動(dòng)進(jìn)入“運(yùn)行”狀態(tài),“運(yùn)行”指示燈亮,儀器便顯示當(dāng)前爐體實(shí)際溫度,并開(kāi)始加溫至設(shè)定值。2、設(shè)定予置參數(shù):在電源燈亮情況下,按下“設(shè)定”開(kāi)關(guān)一次,“設(shè)定”燈亮,三位數(shù)碼顯示設(shè)定值,按下“增加”或“減少”開(kāi)關(guān),直至所需設(shè)定溫度值;“增加”和“減少” 開(kāi)關(guān)按按住不動(dòng)一秒,可快速增減數(shù)值。達(dá)到所需溫度值后,再按一次“運(yùn)行”開(kāi)關(guān),確認(rèn)本次更改有效,設(shè)定溫度值存入機(jī)器內(nèi),同時(shí)進(jìn)入“運(yùn)行”狀態(tài),“運(yùn)行”燈亮,三位數(shù)碼顯示爐體溫度。3、如果要停止工作,再按一下“電源”開(kāi)關(guān),電源指示燈滅,儀器停止工作。并須拔下電源插頭。4、首次工作約 待40分鐘預(yù)熱時(shí)間后,爐體溫度已穩(wěn)定在所需溫度,可進(jìn)行樣品消煮。三。特點(diǎn)1、爐孔溫度連續(xù)可調(diào),可予先設(shè)置并控制溫度恒定,儀器操作簡(jiǎn)單,使用范圍廣。2、爐體結(jié)構(gòu)新穎且采用高輻射遠(yuǎn)紅外材料,升溫速度快,恒溫性能好,熱效率高。3、爐孔間平均溫度偏差小,樣品消化一致性好四。注意事項(xiàng) 1、硫酸回流高度應(yīng)控制在管高的三分之二以下。2、消煮植株、種子等生物試樣時(shí),一定做到加入消化劑后放置4至6個(gè)小時(shí),或過(guò)夜后才能進(jìn)行消煮;實(shí)在做不到此點(diǎn)時(shí),在消煮 過(guò)程中,應(yīng)先將 爐溫控制在100℃以下,消煮半小時(shí)后才升至高溫進(jìn)行消煮 ,以防消煮 過(guò)程中的巨沸或飛濺。3、作結(jié)束,要拔下電源插頭,電源插頭的地線要良好接地,以保證用電安全。4、本機(jī)處于設(shè)定狀態(tài)時(shí),不進(jìn)行控溫運(yùn)算,因此,長(zhǎng)期使機(jī)器處于設(shè)定狀態(tài),將無(wú)法達(dá)到控制目標(biāo);建議用戶在查看、更改設(shè)定值后,應(yīng)立即進(jìn)入“運(yùn)行”狀態(tài)。五。 產(chǎn)品配套清單 1、消 煮 爐 1臺(tái)。2、雙層試管架 1只。3、消 化 管 20或12或35只。

該公司產(chǎn)品分類: 耗材 壓力儀表 瓶類檢測(cè)儀 微型水泵,真空泵 電阻率測(cè)定儀 便攜式電測(cè)水位計(jì) 糧食水分測(cè)定儀 促銷產(chǎn)品 折光儀/折射儀 定氮儀,消煮爐 照度計(jì) 活性炭測(cè)試儀 晶閘管測(cè)試儀 可控硅控制器 焊接檢驗(yàn)尺 無(wú)菌檢查薄膜過(guò)濾器 管樁荷載測(cè)試儀

AN218004DWDM光譜分析儀AN218004 [1526.05—1563.86 nm] SC/PC

DWDM光譜分析儀AN218004 [1526.05—1563.86 nm] SC/PC DWDM光譜分析儀特征:·PLC 設(shè)備· DWDM(96通道)測(cè)量解決方案· 同一時(shí)間自動(dòng)測(cè)量 CWDM-96通道 (波長(zhǎng) & 功率) · CWDM 彩色圖文掃描· 保存和調(diào)用300數(shù)據(jù)· 軟件可以自行保存數(shù)據(jù)· 自動(dòng)關(guān)機(jī)DWDM光譜分析儀技術(shù)參數(shù): 

技術(shù)參數(shù)

通道數(shù)量

96

通道間隔

50GHz, 100GHz

通道頻率

196.45~191.7THz

最大輸入功率

500mW; 27dBm

帶寬

1526.05~1563.86 nm

光源接頭

SC/PC Standard

測(cè)量速度

4 Sec. (all 96ch.)

電池

鋰聚合物電池, 1800毫安時(shí),3.7

測(cè)量范圍

+10~-40dBm

電池工作時(shí)長(zhǎng)

充滿電后單次使用620分鐘

測(cè)量精度

± 1.0dB @ -40 dBm

電流消耗(Max)

0.25A

顯示屏分辨率

0.01dB

電力消耗

0.925W

顯示單位

dB, dBm,nm,THz

顯示

3.5” TFT-LCD, 16bit color, 240*320

重量

0.6 kg

尺寸

196*95*40 mm

溫度

(環(huán)境條件)

-20 to +55 °C (操作環(huán)境)

濕度

(最大無(wú)冷凝)

95%   (操作環(huán)境)

-35 to +65°C (儲(chǔ)存環(huán)境)

85%  (儲(chǔ)存環(huán)境)

http://www.yach.com/Instrument.html   更多光學(xué)儀器儀表
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CSB-E04489h人血管緊張素轉(zhuǎn)化酶2(ACE2)ELISA Kit

1Human angiotensin convertingenzyme 2(ACE2)ELISA KitCatalog No. CSB-E04489h(96 T) This immunoassay kit allows for the in vitro quantitative determination of humanACE2 concentrations in serum, plasma and cell culture supernates. Expiration date six months from the date of manufacture FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.2PRINCIPLE OF THE ASSAYThe microtiter plate provided in this kit has been pre-coated withan antibody specific to ACE2. Standards or samples are thenadded to the appropriate microtiter plate wells with abiotin-conjugated antibody preparation specific for ACE2 andAvidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well.Only those wells that contain ACE2, biotin-conjugated antibodyand enzyme-conjugated Avidin will exhibit a change in color. Theenzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm ± 2 nm. Theconcentration of ACE2 in the samples is then determined bycomparing the O.D. of the samples to the standard curve.DETECTION RANGE0.156 ng/ml-10 ng/ml. The standard curve concentrations usedfor the ELISA’s were 10 ng/ml, 5 ng/ml, 2.5 ng/ml, 1.25 ng/ml,0.625 ng/ml, 0.312 ng/ml, 0.156 ng/ml.3SPECIFICITYThis assay recognizes human ACE2. No significantcross-reactivity or interference was observed.SENSITIVITYThe minimum detectable dose of human ACE2 is typically lessthan 0.04 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD)was defined as the lowest protein concentration that could bedifferentiated from zero.MATERIALS PROVIDEDReagent QuantityAssay plate 1Standard 2Sample Diluent 1 x 20 mlBiotin-antibody Diluent 1 x 10 mlHRP-avidin Diluent 1 x 10 mlBiotin-antibody 1 x 120μlHRP-avidin 1 x 120μlWash Buffer1 x 20 ml(25×concentrate)TMB Substrate 1 x 10 mlStop Solution 1 x 10 ml4STORAGE1. Unopened test kits should be stored at 2-8C upon receiptand the microtiter plate should be kept in a sealed bag. Thetest kit may be used throughout the expiration date of the kit,provided it is stored as prescribed above. Refer to thepackage label for the expiration date.2. Opened test plate should be stored at 2-8C in the aluminumfoil bag with desiccants to minimize exposure to damp air. Thekits will remain stable until the expiring date shown, provided itis stored as prescribed above.3. A microtiter plate reader with a bandwidth of 10 nm or lessand an optical density range of 0-3 OD or greater at 450nmwavelength is acceptable for use in absorbancemeasurement.REAGENT PREPARATIONBring all reagents to room temperature before use.1. Wash Buffer If crystals have formed in the concentrate,warm up to room temperature and mix gently until thecrystals have completely dissolved. Dilute 20 ml of WashBuffer Concentrate into deionized or distilled water to prepare500 ml of Wash Buffer.52. Standard Centrifuge the standard vial at 6000-10000rpmfor 30s. Reconstitute the Standard with 1.0 ml of SampleDiluent. This reconstitution produces a stock solution of 10ng/ml. Allow the standard to sit for a minimum of 15 minuteswith gentle agitation prior to making serial dilutions. Theundiluted standard serves as the high standard (10 ng/ml).The Sample Diluent serves as the zero standard (0 ng/ml).Prepare fresh for each assay. Use within 4 hours and discardafter use.3. Biotin-antibody Centrifuge the vial before opening. Diluteto the working concentration using Biotin-antibodyDiluent(1:100), respectively.4. HRP-avidin Centrifuge the vial before opening. Dilute to theworking concentration using HRP-avidin Diluent(1:100),respectively.Precaution: The Stop Solution provided with this kit is an acid solution. Weareye, hand, face, and clothing protection when using this material.OTHER SUPPLIES REQUIRED Microplate reader capable of measuring absorbance at 450nm, with the correction wavelength set at 540 nm or 570 nm. Pipettes and pipette tips. Deionized or distilled water.6 Squirt bottle, manifold dispenser, or automated microplatewasher. An incubator which can provide stable incubation conditionsup to 37°C±0.5°C.SAMPLE COLLECTION AND STORAGE Serum Use a serum separator tube (SST) and allowsamples to clot for 30 minutes before centrifugation for 15minutes at 1000 g. Remove serum and assay immediately oraliquot and store samples at -20°C. Centrifuge the sampleagain after thawing before the assay. Avoid repeatedfreeze-thaw cycles. Plasma Collect plasma using citrate, EDTA, or heparin asan anticoagulant. Centrifuge for 15 minutes at 1000 g within30 minutes of collection. Assay immediately or aliquot andstore samples at -20°C. Centrifuge the sample again afterthawing before the assay. Avoid repeated freeze-thaw cycles. Cell Culture Supernates Remove particulates bycentrifugation for 15 minutes at 1000 x g, 2 - 8°C and assayimmediately or aliquot and store samples at -20° C or -80°C.Avoid repeated freeze-thaw cycles.Note: Grossly hemolyzed samples are not suitable for use in this assay.7ASSAY PROCEDUREBring all reagents and samples to room temperature before use. It isrecommended that all samples, standards, and controls be assayed in duplicate.All the reagents should be added directly to the liquid level in the well. Thepipette should avoid contacting the inner wall of the well.1. Add 100μl of Standard, Blank, or Sample per well. Cover withthe adhesive strip. Incubate for 2 hours at 37°C.2. Remove the liquid of each well, don’t wash.3. Add 100μl of Biotin-antibody working solution to each well.Incubate for 1 hour at 37°C. Biotin-antibody workingsolution may appear cloudy. Warm up to room temperatureand mix gently until solution appears uniform.4. Aspirate each well and wash, repeating the process threetimes for a total of three washes. Wash: Fill each well withWash Buffer (200μl) and let it stand for 2 minutes, thenremove the liquid by flicking the plate over a sink. Theremaining drops are removed by patting the plate on a papertowel. Complete removal of liquid at each step is essential togood performance.5. Add 100μl of HRP-avidin working solution to each well.Cover the microtiter plate with a new adhesive strip. Incubatefor 1 hour at 37°C.6. Repeat the aspiration and wash five times as step 4.87. Add 90μl of TMB Substrate to each well. Incubate for 10-30minutes at 37°C. Keeping the plate away from drafts andother temperature fluctuations in the dark.8. Add 50μl of Stop Solution to each well when the first fourwells containing the highest concentration of standardsdevelop obvious blue color. If color change does not appearuniform, gently tap the plate to ensure thorough mixing.9. Determine the optical density of each well within 30 minutes,using a microplate reader set to 450 nm.CALCULATION OF RESULTSUsing the professional soft "Curve Exert 1.3" to make a standard curve isrecommended, which can be downloaded from our web.Average the duplicate readings for each standard, control, andsample and subtract the average zero standard optical density.Create a standard curve by reducing the data using computersoftware capable of generating a four parameter logistic (4-PL)curve-fit. As an alternative, construct a standard curve by plottingthe mean absorbance for each standard on the x-axis againstthe concentration on the y-axis and draw a best fit curve throughthe points on the graph. The data may be linearized by plottingthe log of the ACE2 concentrations versus the log of the O.D.and the best fit line can be determined by regression analysis.9This procedure will produce an adequate but less precise fit ofthe data. If samples have been diluted, the concentration readfrom the standard curve must be multiplied by the dilution factor.LIMITATIONS OF THE PROCEDURE The kit should not be used beyond the expiration date on thekit label. Do not mix or substitute reagents with those from other lots orsources. It is important that the Standard Diluent selected for thestandard curve be consistent with the samples beingassayed. If samples generate values higher than the highest standard,dilute the samples with the appropriate Standard Diluent andrepeat the assay. Any variation in Standard Diluent, operator, pipettingtechnique, washing technique, incubation time ortemperature, and kit age can cause variation in binding. This assay is designed to eliminate interference by solublereceptors, binding proteins, and other factors present inbiological samples. Until all factors have been tested in theImmunoassay, the possibility of interference cannot beexcluded.1 0TECHNICAL HINTS Centrifuge vials before opening to collect contents. When mixing or reconstituting protein solutions, always avoidfoaming. To avoid cross-contamination, change pipette tips betweenadditions of each standard level, between sample additions,and between reagent additions. Also, use separate reservoirsfor each reagent. When using an automated plate washer, adding a 30 secondsoak period following the addition of wash buffer, and/orrotating the plate 180 degrees between wash steps mayimprove assay precision. To ensure accurate results, proper adhesion of plate sealersduring incubation steps is necessary. Substrate Solution should remain colorless or light blue untiladded to the plate. Keep Substrate Solution protected fromlight. Substrate Solution should change from colorless or lightblue to gradations of blue. Stop Solution should be added to the plate in the same orderas the Substrate Solution. The color developed in the wellswill turn from blue to yellow upon addition of the Stop Solution.Wells that are green in color indicate that the Stop Solutionhas not mixed thoroughly with the Substrate Solution.

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